sirtuin 3 (sirt3: #5490) antibody  (Cell Signaling Technology Inc)

Journal: Life

Article Title: Supplementation of Nicotinic Acid and Its Derivatives Up-Regulates Cellular NAD + Level Rather than Nicotinamide Derivatives in Cultured Normal Human Epidermal Keratinocytes

doi: 10.3390/life14030413

Figure Lengend Snippet: Ablation of mitochondrial ROS by the supplementation of nicotinic acid ( A ) Representative images of the effects of NA on the mitochondrial markers superoxide dismutase (SOD) 2 and sirtuin 3 (SIRT3). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is shown as the loading control. ( B , C ) Quantification of SOD2 ( B ) and SIRT3 ( C ) immunoblot bands on ( A ). The data are divided by the intensity of GAPDH. Individual quantified values are represented as gray dots on the bar chart. ( D ) Representative images of the effect of NA on mitochondrial ROS recovery activity. Mitochondria and mitochondrial ROS were stained by green and red fluorescent dye, and 50 μM mito-TEMPO was used as a positive control. R: 1 μM rotenone. The red/green pixel ratio (R/G ratio) is depicted by a histogram, and the means of the histograms are shown in ( E ). All data are expressed as the mean ± SE of at least three independent experiments. The statistical analyses were performed by a one-way ANOVA followed by Dunnett’s test. * p < 0.05.

Article Snippet: The superoxide dismutase 2 (SOD2) and sirtuin 3 (SIRT3) antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Control, Western Blot, Activity Assay, Staining, Positive Control